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sirna targeting the 5-ht 2a r  (Shanghai GenePharma)
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Shanghai GenePharma sirna targeting the 5-ht 2a r
Ketanserin inhibits the expression of pro-inflammatory cytokines and influences the polarization of macrophages partly <t>through</t> <t>5-hydroxytrypta-mine-2A</t> receptor (5-HT 2A R)/nuclear factor-κB (NF-κB). Bone marrow-derived macrophages (BMDMs) were transfected with control <t>siRNA</t> or 5-HT 2A R siRNA followed by stimulation with LPS plus 5-HT (LPS/5-HT). Relative mRNA level expression of (A) interleukin (IL)-1β, (B) necrosis factor-α (TNF-α), (C) IL-6 and (D) iNOS was evaluated in BMDMs by RT-qPCR. (E) Changes in pNF-κB p65 levels in LPS/5-HT-treated BMDMs were evaluated by western blot analyses. (F) Protein levels of pNF-κB p65 were quantified by densitometric analysis. The graphs depict the means ± SD of 3 independent experiments. * p<0.05 and ** p<0.01 vs. LPS/5HT with ket-treated BMDMs.
Sirna Targeting The 5 Ht 2a R, supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sirna targeting the 5-ht 2a r/product/Shanghai GenePharma
Average 90 stars, based on 1 article reviews
sirna targeting the 5-ht 2a r - by Bioz Stars, 2026-03
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sirna targeting 5-ht 2a r  (Shanghai GenePharma)
90
Shanghai GenePharma sirna targeting 5-ht 2a r
Sequences of primers used for RT-qPCR.
Sirna Targeting 5 Ht 2a R, supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sirna targeting 5-ht 2a r/product/Shanghai GenePharma
Average 90 stars, based on 1 article reviews
sirna targeting 5-ht 2a r - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier

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Ketanserin inhibits the expression of pro-inflammatory cytokines and influences the polarization of macrophages partly through 5-hydroxytrypta-mine-2A receptor (5-HT 2A R)/nuclear factor-κB (NF-κB). Bone marrow-derived macrophages (BMDMs) were transfected with control siRNA or 5-HT 2A R siRNA followed by stimulation with LPS plus 5-HT (LPS/5-HT). Relative mRNA level expression of (A) interleukin (IL)-1β, (B) necrosis factor-α (TNF-α), (C) IL-6 and (D) iNOS was evaluated in BMDMs by RT-qPCR. (E) Changes in pNF-κB p65 levels in LPS/5-HT-treated BMDMs were evaluated by western blot analyses. (F) Protein levels of pNF-κB p65 were quantified by densitometric analysis. The graphs depict the means ± SD of 3 independent experiments. * p<0.05 and ** p<0.01 vs. LPS/5HT with ket-treated BMDMs.

Journal: International Journal of Molecular Medicine

Article Title: Effects of ketanserin on experimental colitis in mice and macrophage function

doi: 10.3892/ijmm.2016.2486

Figure Lengend Snippet: Ketanserin inhibits the expression of pro-inflammatory cytokines and influences the polarization of macrophages partly through 5-hydroxytrypta-mine-2A receptor (5-HT 2A R)/nuclear factor-κB (NF-κB). Bone marrow-derived macrophages (BMDMs) were transfected with control siRNA or 5-HT 2A R siRNA followed by stimulation with LPS plus 5-HT (LPS/5-HT). Relative mRNA level expression of (A) interleukin (IL)-1β, (B) necrosis factor-α (TNF-α), (C) IL-6 and (D) iNOS was evaluated in BMDMs by RT-qPCR. (E) Changes in pNF-κB p65 levels in LPS/5-HT-treated BMDMs were evaluated by western blot analyses. (F) Protein levels of pNF-κB p65 were quantified by densitometric analysis. The graphs depict the means ± SD of 3 independent experiments. * p<0.05 and ** p<0.01 vs. LPS/5HT with ket-treated BMDMs.

Article Snippet: siRNA targeting the 5-HT 2A R (Genepharm Biotech, Shanghai, China) was synthesized (GACAACUGUCGUGAUUAUUTT) and control siRNA (UUCUCCGAACGUGUCACGUTT) was also used.

Techniques: Expressing, Derivative Assay, Transfection, Control, Quantitative RT-PCR, Western Blot

Sequences of primers used for RT-qPCR.

Journal: International Journal of Molecular Medicine

Article Title: Effects of ketanserin on experimental colitis in mice and macrophage function

doi: 10.3892/ijmm.2016.2486

Figure Lengend Snippet: Sequences of primers used for RT-qPCR.

Article Snippet: siRNA targeting the 5-HT 2A R (Genepharm Biotech, Shanghai, China) was synthesized (GACAACUGUCGUGAUUAUUTT) and control siRNA (UUCUCCGAACGUGUCACGUTT) was also used.

Techniques:

5-Hydroxytryptamine-2A receptor (5-HT 2A R) expression is upregulated in the colons of patients with inflammatory bowel disease (IBD) and in mice with dextran sodium sulfate (DSS)-induced colitis, and is specifically enhanced in macrophages. (A) Relative mRNA expression of 5-HT 2A R in healthy subjects and patients wtih IBD estimated by RT-qPCR. (B) Representative images of immunofluorescence staining of colonic mucosa samples of healthy subjects and in patients with IBD showing 5-HT 2A R + cells (in green) and CD68 + cells (in red). Co-localization of 5-HT 2A R with the macrophage marker, CD68 + , is shown in the merged images. 5-HT 2A R expression in mice with DSS-induced colitis estimated by (C) RT-qPCR and (D) western blot analysis. Representative images of immunofluorescence staining of 5-HT 2A R + cells (in green) and CD68 + cells (in red) in the colonic mucosa samples of mice treated with or without DSS. (E) Co-localization of 5-HT 2A R with CD68 is shown in the merged images.

Journal: International Journal of Molecular Medicine

Article Title: Effects of ketanserin on experimental colitis in mice and macrophage function

doi: 10.3892/ijmm.2016.2486

Figure Lengend Snippet: 5-Hydroxytryptamine-2A receptor (5-HT 2A R) expression is upregulated in the colons of patients with inflammatory bowel disease (IBD) and in mice with dextran sodium sulfate (DSS)-induced colitis, and is specifically enhanced in macrophages. (A) Relative mRNA expression of 5-HT 2A R in healthy subjects and patients wtih IBD estimated by RT-qPCR. (B) Representative images of immunofluorescence staining of colonic mucosa samples of healthy subjects and in patients with IBD showing 5-HT 2A R + cells (in green) and CD68 + cells (in red). Co-localization of 5-HT 2A R with the macrophage marker, CD68 + , is shown in the merged images. 5-HT 2A R expression in mice with DSS-induced colitis estimated by (C) RT-qPCR and (D) western blot analysis. Representative images of immunofluorescence staining of 5-HT 2A R + cells (in green) and CD68 + cells (in red) in the colonic mucosa samples of mice treated with or without DSS. (E) Co-localization of 5-HT 2A R with CD68 is shown in the merged images.

Article Snippet: siRNA targeting the 5-HT 2A R (Genepharm Biotech, Shanghai, China) was synthesized (GACAACUGUCGUGAUUAUUTT) and control siRNA (UUCUCCGAACGUGUCACGUTT) was also used.

Techniques: Expressing, Quantitative RT-PCR, Immunofluorescence, Staining, Marker, Western Blot

Ketanserin inhibits the expression of pro-inflammatory cytokines and influences the polarization of macrophages partly through 5-hydroxytrypta-mine-2A receptor (5-HT 2A R)/nuclear factor-κB (NF-κB). Bone marrow-derived macrophages (BMDMs) were transfected with control siRNA or 5-HT 2A R siRNA followed by stimulation with LPS plus 5-HT (LPS/5-HT). Relative mRNA level expression of (A) interleukin (IL)-1β, (B) necrosis factor-α (TNF-α), (C) IL-6 and (D) iNOS was evaluated in BMDMs by RT-qPCR. (E) Changes in pNF-κB p65 levels in LPS/5-HT-treated BMDMs were evaluated by western blot analyses. (F) Protein levels of pNF-κB p65 were quantified by densitometric analysis. The graphs depict the means ± SD of 3 independent experiments. * p<0.05 and ** p<0.01 vs. LPS/5HT with ket-treated BMDMs.

Journal: International Journal of Molecular Medicine

Article Title: Effects of ketanserin on experimental colitis in mice and macrophage function

doi: 10.3892/ijmm.2016.2486

Figure Lengend Snippet: Ketanserin inhibits the expression of pro-inflammatory cytokines and influences the polarization of macrophages partly through 5-hydroxytrypta-mine-2A receptor (5-HT 2A R)/nuclear factor-κB (NF-κB). Bone marrow-derived macrophages (BMDMs) were transfected with control siRNA or 5-HT 2A R siRNA followed by stimulation with LPS plus 5-HT (LPS/5-HT). Relative mRNA level expression of (A) interleukin (IL)-1β, (B) necrosis factor-α (TNF-α), (C) IL-6 and (D) iNOS was evaluated in BMDMs by RT-qPCR. (E) Changes in pNF-κB p65 levels in LPS/5-HT-treated BMDMs were evaluated by western blot analyses. (F) Protein levels of pNF-κB p65 were quantified by densitometric analysis. The graphs depict the means ± SD of 3 independent experiments. * p<0.05 and ** p<0.01 vs. LPS/5HT with ket-treated BMDMs.

Article Snippet: siRNA targeting the 5-HT 2A R (Genepharm Biotech, Shanghai, China) was synthesized (GACAACUGUCGUGAUUAUUTT) and control siRNA (UUCUCCGAACGUGUCACGUTT) was also used.

Techniques: Expressing, Derivative Assay, Transfection, Quantitative RT-PCR, Western Blot